FastQCFastQC Report
Mon 27 Feb 2023
SRR3126977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803314
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96791.2048837689869716No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36750.457479889557508No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG35960.4476456279860677No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG28800.35851485222465934No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC26410.3287630988629602No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT11380.14166316035821608No Hit
AAAGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT10990.13680827173434051No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10480.1304595712261955No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT10080.12548019827863077No Hit
GGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT9890.12311499612853753No Hit
AGAGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT9090.1131562502334081No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCACC8120.10108127083556367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2300.088.089442
ACGGGTA700.087.4986654
TACGGGT650.086.980933
CGTTTTT31300.086.5903241
CGGGTAT550.085.444425
CGCTACG6350.085.325773
TAGGGTA2700.083.75944
CTACGAC6650.082.681935
AGGGCGT1100.081.17225
ACGGGAT2850.079.351014
AGGGATG16550.079.2232365
GCTACGA6950.077.959524
TAGGGTC1900.076.871294
CGACCAA6850.076.837618
AAGGGCG2650.076.450214
CGAAGGG6550.076.2513962
TACGACC7150.076.2427146
AGGGCAT8150.076.113685
GGGTATC3100.075.797476
TCCGCTA7250.075.4164051
Produced by FastQC (version 0.11.5)