FastQCFastQC Report
Mon 27 Feb 2023
SRR3126976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823177
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99461.2082456142482114No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40060.48665110905673997No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG33750.4099968779496998No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG27710.3366226218662572No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC25110.3050376771945766No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11670.14176780935327396No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT10500.1275545842510177No Hit
GGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT10420.12658273979958137No Hit
AAAGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT10050.12208795921168837No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT9760.11856502307523169No Hit
AGAGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT8870.10775325355300257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA156.9028226E-493.9955147
GCGATTG353.583409E-1093.995518
CGTTTTT30450.086.8891141
CGTAGGG2650.085.174572
TAAGCGG1000.084.7400741
AGGGATG18000.081.2016755
CGCTACG7950.079.807513
GCGTAGG1600.079.4438251
AGAGGGC11750.077.996273
TCCGCTA8300.077.7067641
TAGAGGG13550.077.042122
GACCGAT551.8189894E-1276.905418
GCTACGA8250.076.905414
ACGGGAT3000.076.7634
CTACGAC8300.076.442135
AAGGGTA4000.076.3713464
TAAGGGA7550.075.943393
CGAAGGG8450.075.6827242
TACGCGG255.513049E-575.324521
CGGGTAT255.5597586E-575.19645
Produced by FastQC (version 0.11.5)