FastQCFastQC Report
Mon 27 Feb 2023
SRR3126975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432144
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182201.2722184361349138No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG46330.3235009887273905Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG39720.2773464120926387RNA PCR Primer, Index 41 (96% over 25bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36250.25311700499391127No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC34980.24424918164653833RNA PCR Primer, Index 41 (96% over 26bp)
AAAGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT15890.11095252991319308RNA PCR Primer, Index 41 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT15370.10732161011741835RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52550.086.5996551
ATAGCGG2700.081.836611
TCCGCTA6900.075.628841
GGTAAGG9950.075.598021
TAGCGGG7250.074.550752
AGGGATG21400.074.0130545
AGTAGGG21650.072.7242362
TACGACC7200.071.804636
AGGGAAT21950.071.516165
CTACGAC7300.071.464825
AGGGTAC9350.071.378755
AGGGTAT9000.070.4990845
TAGGGCG2400.070.4990844
ATAGAGG8450.070.101521
TAAGAGG10050.069.700151
ATAGGGA14200.069.5061343
CGCTACG7450.069.395073
GTAAGGG20350.068.5937042
TAGGGTC2200.068.362754
AGGGCAT11900.068.326845
Produced by FastQC (version 0.11.5)