SRR3126974.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3126974.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1153320
Sequences flagged as poor quality	0
Sequence length	100
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13118	1.1374119932022335	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	4383	0.38003329518260326	Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	3562	0.3088475011271807	RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	3283	0.28465647001699446	RNA PCR Primer, Index 41 (96% over 26bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	2657	0.23037838587729337	No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	1425	0.12355634169181147	RNA PCR Primer, Index 41 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1365	0.11835396941005098	RNA PCR Primer, Index 41 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1333	0.11557937085977872	RNA PCR Primer, Index 41 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1242	0.10768910623244199	RNA PCR Primer, Index 41 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3990	85.679306	1
CTACGAC	550	78.60766	5
AGGGAAT	1630	76.689125	5
CGCTACG	560	76.590576	3
CGTAGGG	265	76.482735	2
GCTACGA	575	75.41227	4
TACGACC	575	75.18995	6
GCGCATA	75	75.18994	8
TAGCGGG	550	74.55854	2
AGGGTAC	760	73.58226	5
TGTAGGG	580	73.140076	2
ATAGCGG	200	73.0874	1
AGGGCAT	760	72.96392	5
AGTAGGG	1715	72.55722	2
GGTAAGG	765	72.11661	1
AGGGATG	1795	71.99594	5
AGGGTAT	800	71.66541	5
GTAGGGA	975	71.54497	3
TAGGGCG	165	71.413124	4
GAATAGG	575	71.34479	1