FastQCFastQC Report
Mon 27 Feb 2023
SRR3126972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641179
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288351.091747284072757No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG58900.22300646794480797No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG45670.1729152018852187No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC45050.1705677653805365No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31000.11737182523410947No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28120.10646760405106963No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26520.10040970339382527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84600.084.862431
ATAGGGC15600.075.016543
TAGGGCA12150.073.8849264
CGTAGGG6400.073.434872
ATAGCGG4650.072.799151
AGTAGGG39400.072.048172
TAGCGGG13500.071.367812
AGGGATG33150.070.7469255
ACTAGGG13250.070.586142
GAATAGG14100.070.0241
AGGGTAC12900.069.95235
AGGGCAT24200.069.9161765
GGTAAGG14550.069.1508561
AGGGAAT32900.067.998855
AATAGGG35600.067.9891362
AGATAGG14100.067.6898651
AGAGGGC37700.067.567863
ATAAGGG36150.067.344752
ATAGGGA25050.067.167253
AAGGGCA38500.066.53264
Produced by FastQC (version 0.11.5)