FastQCFastQC Report
Mon 27 Feb 2023
SRR3126970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190021
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219041.000173057701273No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG57240.2613673567513736No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC39560.1806375372656244No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG38990.17803482249713587No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24290.11091217846769506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69650.083.494391
CGTAGGG5000.079.922942
AGGGATG28350.077.085675
GAATAGG12100.075.824431
CGGGAAT4500.073.107065
TAGGGCA8600.072.682024
AGGGCAT18250.072.10565
ATAGGGC10900.072.005193
AGTAGGG29750.071.428912
GTAGGGT7350.070.975663
AGGGTAC8450.070.635145
TAGGGAT14750.070.4164354
ACGGGAT3900.069.893574
TAGGGTA6750.069.625784
ATAAGGG29600.068.455472
AATAGGG28000.068.169572
TAGCGGG11000.067.528482
GTAGGGA16450.067.4248353
ATAGGGA19550.067.310853
GGTAAGG12250.067.214331
Produced by FastQC (version 0.11.5)