FastQCFastQC Report
Mon 27 Feb 2023
SRR3126969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219208
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT877692.726415938330173No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT71030.22064433239480022No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT64480.20029771297785043No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48810.15162114408264393No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT48250.14988158578134747TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG44390.13789105891883968No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC33230.10322414705728862TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT273800.089.649061
TAGGGCA16300.074.972724
AGTAGGG51000.071.513632
AGGGATG45550.070.8890465
GTAGGGC17700.069.04163
CGTAGGG9700.068.3194962
ACGGGAT8600.068.317054
GAATAGG18900.067.908841
ATAGGGC19550.067.797413
TAGAGGG58050.067.119642
TGTAGGG20350.066.746852
GTAGGGA23450.066.743993
GGTAAGG18750.066.446191
ATAGGGA26000.066.1635063
AGGGACT41350.065.358655
AGATAGG15500.065.212711
AAGAGGG105500.064.908922
AGAGGGC46700.064.714993
AGGGCAT26450.064.682455
GAGGGAT37600.064.5029144