FastQCFastQC Report
Mon 27 Feb 2023
SRR3126966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3827594
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524011.3690323477359407No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48970.12793937915045328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT187900.087.458631
ACGGGAT14650.077.008164
ATAGGGC26800.074.885793
AGGGATG85000.072.773245
TAGGGCA23550.072.456744
AGTAGGG62300.069.782852
CGAAGGG36900.068.780042
GGATGGC59600.068.692087
TAGAGGG72900.068.3397452
AGGGAAT49450.068.1534045
AGAGGGC58550.067.992793
GAGGGAT63250.067.482084
CGGGATG17650.067.376825
TAAGGGA39600.067.296773
AAGGGAC42250.067.089374
ATAGGGA35350.066.6123353
ATAAGGG55450.066.367472
AGGGCAT36650.066.305775
GGAATGC32100.065.893457
AAGGGAT78600.065.187914
Produced by FastQC (version 0.11.5)