FastQCFastQC Report
Mon 27 Feb 2023
SRR3126965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008317
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391351.3008934896156223No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37010.12302559869854142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140950.086.8385851
ATAGGGC20100.074.5604863
TAGGGCA18850.073.004424
AGGGATG65200.072.725245
ATAGCGG6300.072.59461
ACGGGAT10250.072.186084
GTAGGGC16200.070.400863
TAGAGGG53950.070.146992
AGGGCAT30350.070.142475
GGATGGC51300.069.8954247
AGAGGGC44500.069.791583
AGTAGGG54600.069.225582
AGGGAAT38700.068.730115
GGAATGC26000.067.779687
TAAGGGA29000.067.6040953
ATAACGG2900.066.659031
GTAGGGT14500.066.6290363
TAGGGCG5950.066.533444
TATAGGG23600.066.099912
CGAAGGG27850.065.997022
Produced by FastQC (version 0.11.5)