FastQCFastQC Report
Mon 27 Feb 2023
SRR3126964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113695
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400401.2859319875581905No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38190.12265170480731091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT141300.086.363041
ATAGGGC20400.074.643013
ACGGGAT10450.073.7567754
AGGGATG65400.073.658095
TAGGGCA19650.072.7085344
AGTAGGG50400.070.4323352
GTAGGGC16000.069.9087143
TAGGGCG5650.069.872324
ATAGCGG6900.069.569461
CGAAGGG31850.068.791092
AGAGGGC48650.068.008653
AGGGCAT29500.067.8675165
TAGAGGG56900.067.674852
AAGGGAT65150.067.30414
GTAGGGT12750.067.086563
ATAGAGG22750.066.196661
ATAAGGG44750.066.191652
TAAGGGA32300.065.912843
GAGGGAT46800.065.6759344
AGGGAAT39750.065.264395
Produced by FastQC (version 0.11.5)