FastQCFastQC Report
Mon 27 Feb 2023
SRR3126962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318294
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT617902.665321999711857No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT61210.2640303602562919No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT54160.23362006717008282No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT47790.20614296547374922No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG35180.15174951925855823No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC26360.11370430152517325No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTC25650.11064170463280325No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC25150.10848494625789482No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24120.10404202400558342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT195900.089.01371
AGGGATG36450.075.681055
ATAGGGC13650.073.166053
TAGAGGG38400.069.562792
TAGGGCA11850.069.17314
GGTAAGG12000.069.141431
ATAGGGA18800.068.6592563
GAGGGAT25950.067.169294
AGAGGGC32350.066.841283
TAGGGAT13900.066.766374
AGTACGG2900.066.648881
CGTAGGG6650.066.593422
TAAGAGG16500.066.284351
GTACGGG5050.066.23572
GTAGGGA17400.066.061283
AGTAGGG37200.065.474592
TATAGGG16600.065.2747042
ATAGCGG4550.065.2733841
GAATAGG12300.065.155451
GTAGGGC11750.064.950653