FastQCFastQC Report
Mon 27 Feb 2023
SRR3126960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524883
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402771.1426478552621462No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT54620.1549554978136863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT136850.085.0338441
ATAGGGC25100.076.020173
TAGGGCA22550.075.868624
ATAGCGG9300.073.3071751
CGTAGGG11400.072.145582
AGTAGGG53500.071.594622
AGGGATG54950.071.334495
AGGGCAT31700.070.426415
GTAGGGA26400.069.606443
ACGGGAT11200.069.242594
GAATAGG21100.068.855311
CGAAGGG38150.068.248292
AGATAGG23400.068.115391
TAAGGGA30000.067.993
GCATAGG10900.067.722751
TAGGGAT26750.067.4706344
ATAGGGA32350.067.409363
AATAGGG52850.066.694932
GATAGGG56700.066.559342
ATAAGGG48900.066.123542
Produced by FastQC (version 0.11.5)