FastQCFastQC Report
Mon 27 Feb 2023
SRR3126959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728382
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300081.0998459893079486No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41360.15159167594567036No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG29100.10665661919775163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101700.086.079191
ACGGGTA2400.076.576044
TAGGGCA16050.076.043894
ATAGGGC18450.073.814393
ATAGCGG6200.073.754281
CGTAGGG7500.072.884692
ACGGGAT7400.071.322394
AGTAGGG43600.071.009822
GTAGGGA22350.070.0003363
GGTAAGG16900.069.457611
GTACGGG6250.069.366122
TAGGGAT18700.068.795594
CGAAGGG28350.068.483152
AGGGATG40700.068.355175
AGGGCAT24050.067.413695
ATAGGGA25450.067.21383
GTAGGGC13100.066.9084853
GATAGGG40800.066.87412
AAGGGAC28550.066.51794
AGTAAGG18800.066.450041
Produced by FastQC (version 0.11.5)