FastQCFastQC Report
Mon 27 Feb 2023
SRR3126958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823564
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309891.0975136387912583No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45430.16089594569133195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102950.085.9834061
TAGGGCA17050.075.251184
ATAGCGG6500.074.5720441
ACGGGAT8650.074.4353944
ATAGGGC19050.071.7915043
CGTAGGG9650.071.1390842
AGTAGGG44550.070.9258042
AGGGATG44050.070.736355
GTAGGGA21950.069.800433
GGTAAGG17700.069.6594851
GACCGAT1500.068.929718
CGAAGGG30550.068.7985152
ATAGGGA27500.067.6764453
AGTAAGG20750.067.5850141
AGGGCAT26350.067.062885
GTAGGGC15450.066.617863
TAAGGGA25150.066.525333
AAGGGAT46800.066.378994
AGGGAAT33300.065.909455
ATGCGGG14700.065.891992
Produced by FastQC (version 0.11.5)