FastQCFastQC Report
Mon 27 Feb 2023
SRR3126957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947852
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143841.5175364930389976No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59190.6244645788583028No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG38110.40206698936120827No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG33980.35849478610584773No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC29770.31407856922810734No Hit
AAAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT14950.15772504568223733No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT13530.1427438038849947No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT11780.12428100589543516No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC9980.10529069939188818No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT9870.10413018066111586No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.10370817384992594No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCCT9800.1033916687415335No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT9670.1020201466051662No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT9650.10180914319957125No Hit
TAGACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT9550.1007541261715964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT650.086.7802353
CGTTTTT46000.086.138991
CGCTACG10500.083.715383
ACGGGAT3700.082.578044
CGAAGGG7800.080.745312
GCTACGA10900.080.643264
TAACGGG2400.080.293382
AGGGAAT17150.079.755185
TCCGCTA11350.079.098371
TAGCGGG3300.078.335012
GGATGGC18300.078.082287
ATAGCGG1750.077.891491
AGGGATG23800.077.8124855
CTACGAC11300.077.784525
AGTACGG1150.077.657931
AGGGCAT10050.077.169895
TAGGGCA5000.077.089784
AAGGGCA11850.076.95494
AAGGGAT18550.076.780634
GATGGCA17050.076.363438
Produced by FastQC (version 0.11.5)