FastQCFastQC Report
Mon 27 Feb 2023
SRR3126956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776185
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102861.3251995336163414No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43040.5545069796504699No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG35830.4616167537378331No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG30830.39719912134349417No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC28680.3694995394139284No Hit
AAAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT13530.17431411325908128No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT12640.16284777469288894No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT11710.1508660950675419No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT11630.14983541294923244No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT9430.12149165469572332No Hit
TAGACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT9040.11646707936896487No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCCT9020.11620940883938752No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG8770.11298852721967056No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC8070.1039700586844631No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT8050.10371238815488576No Hit
GGAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT7850.10113568285911219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG900.089.075421
CGTTTTT31000.087.621811
GACCGAT700.087.273688
CGCTACG7250.085.181323
CGAGGGA4300.084.417673
GCTACGA7450.082.894584
TCCGCTA7800.081.014291
AGTACGG700.080.841551
CGAAGGG5950.080.0231252
AGGGAAT13500.079.3668445
AGGGATG19550.079.324125
GGATGGC15800.077.331127
GCATAGG2150.076.768141
ACGACCA7850.076.626397
CTACGAC8050.076.473945
CGTAGGG1300.076.153012
CGACCAA7900.076.141418
TAACGGG1550.076.0360262
AGGGTAC2600.075.912615
AGGGCAC5450.075.8794565
Produced by FastQC (version 0.11.5)