FastQCFastQC Report
Mon 27 Feb 2023
SRR3126955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791291
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106471.3455226964542755No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45150.5705865478060537No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG35300.44610642608092343No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG28860.36472043786672664No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC28400.358907152994284No Hit
AAAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT13890.17553592799614806No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT12740.1610027158150415No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT11470.144952994536776No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT11310.1429309824072307No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT9250.11689757623933547No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCCT9010.11386455804501758No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC8860.1119689216735689No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG8620.108935903479251No Hit
TAGACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT8600.10868315196305785No Hit
GGAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT7940.10034235192868363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT34700.087.182771
GTACGGG1450.084.310152
CGCTACG8950.083.494233
GCTACGA8950.083.494234
CGAAGGG6550.082.552662
CGACCAA8900.080.7948768
AGGGCAC5650.080.687395
AGGGAAT14600.080.154685
ACGGGTG1650.079.754744
AAGGGCG2950.079.658184
TCCGCTA9400.079.5826641
TACGGGT950.079.155083
AGGGTAT4750.079.155075
AGGGATG17400.078.870755
CTACGAC9350.078.414325
GGGCGAT2700.078.330546
CGGGTAT600.078.3305365
GGAATGC11000.078.1881267
CGGGAAT2850.077.5060045
ACGACCA9400.077.4972467