FastQCFastQC Report
Mon 27 Feb 2023
SRR3126954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907524
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383670.9818749673706418No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC82460.21102877423145708No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG39880.10205951390189798No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC39350.10070315626980154No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG39260.1004728313888795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148500.082.7576751
CGTAGGG11750.078.3976062
AGGGATG77750.074.656535
ATAGGGC26250.072.334883
ACGGGAT12750.070.779754
AGTAGGG63700.070.092062
ATAGCGG8550.069.833971
TAGGGCA25250.069.6190644
TAGCGGG20650.069.189172
AAGGGAT82800.068.8570564
TAGGGCG6500.068.695484
TAGAGGG71500.068.295822
AGAGGGC55700.068.010443
AAGGGTA30900.067.8413244
TAGGGAT34650.067.82424
GTAGGGC20450.067.569353
GTAGGGA36300.067.456963
ATAGGGA44750.067.4276053
GAGGGAT68550.067.2635044
TAACGGG12250.067.140812
Produced by FastQC (version 0.11.5)