FastQCFastQC Report
Mon 27 Feb 2023
SRR3126953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124081
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281120.8998486274843707No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62830.20111514394153032No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG37910.12134768592747755No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC34650.11091261718246101No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG34250.10963224064933015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107550.082.101731
ATAGGGC21250.075.608293
TAGGGCA20550.072.910364
TAGGGCG4600.071.699174
CGTAGGG7650.071.449362
AGGGCAT31250.071.431555
GTAGGGC14550.070.593953
AGGGATG58250.070.26985
ATAGCGG7350.069.913991
ATAGAGG21450.069.8915561
TAGAGGG53600.069.888252
AAGGGAT66000.069.532684
CTACGAC13550.069.364495
GTACGGG8250.069.108782
AGTAGGG48900.068.896792
AGAGGGC45650.068.739663
TAAGGGA32500.068.717753
TACGACC13650.068.512046
AGGGATC27400.068.433515
TAGCGGG16750.068.358632
Produced by FastQC (version 0.11.5)