FastQCFastQC Report
Mon 27 Feb 2023
SRR3126952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261213
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288710.8852840952124255No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66980.20538370232180478No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG35710.10949913421785085No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG32640.10008545899945819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115100.082.2531741
AGGGATG63100.072.4698265
ACGGGAT11500.071.926434
ATAGGGC21800.071.789583
AGTAGGG57950.070.6553342
TAGGGCA20350.070.438344
ATAGCGG7700.070.276021
TAAGGGA33300.070.002113
TAGAGGG59250.069.501792
AAGGGAT66350.069.486724
GTAGGGC15650.069.369943
AGGGTAC16950.069.0404055
CGTAGGG9400.069.013092
CGAAGGG28600.068.540972
AGGGATC28550.068.479555
CGAGGGA20900.068.1349643
AAGGGCA53700.067.739254
ATAGGGA37200.067.716673
AGAGGGA82700.067.6262363
AAGGGAC36500.067.470224
Produced by FastQC (version 0.11.5)