FastQCFastQC Report
Mon 27 Feb 2023
SRR3126950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242981
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131951.061560876634478No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC99210.7981618383547294No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG42970.34570118127308463No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC36860.29654516038459156No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG32190.2589741918822573No Hit
AAAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT23880.19211878540379942No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGC21890.17610888662014948No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC19170.15422600989073848No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT13980.11247155024895795No Hit
TAGACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT12430.10000152858330096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40850.086.806721
CGCTACG17450.084.5475853
ACGGGTA1900.084.348554
TCCGCTA17750.083.417821
GCTACGA17650.083.322484
ACGGGAT5700.082.694664
TACGCGG401.0095391E-982.521221
AGGGATG43150.082.334355
GGATGGC31850.081.298447
CGGGATG8500.080.718675
TAGGGCG2000.080.131134
AGGGAAT24950.080.049465
CACGGGC1300.079.768543
GAATGCC18000.079.628298
ACGACCA17800.079.202947
CGAAGGG10250.079.0996252
TACGACC18450.078.704976
AGGGTAT7950.078.618475
CTACGAC18600.078.575565
CGGGTAT1500.078.3229145