FastQCFastQC Report
Mon 27 Feb 2023
SRR3126949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282353
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136241.0624219696136712No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC104940.8183394120027794No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG41850.3263531960388442No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC37370.29141741782488906No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG29850.23277521868003584No Hit
AAAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT22890.178499991811927No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGC22180.17296329481819747No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC20630.16087613940935142No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT13430.10472935299406638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39650.087.33011
AGGGATG39200.084.523015
CGCTACG18500.082.054563
TCCGCTA18900.081.39831
GCTACGA18800.080.745184
GGATGGC32650.080.3198557
ACGGGTA2500.078.9552844
CTACGAC19350.078.692985
TACGACC19350.078.692986
ACGGGAT6600.078.328654
ACGACCA18850.078.037777
TACGCGG850.077.488721
AGGGAAT25050.077.2967765
GGATGAC25100.077.14287
CGAGGGA6950.076.4127043
AGGGCAT13900.076.4127045
AAGGGCG4750.076.184924
GATGGCA32000.075.7829748
GAATGCC18300.075.246878
AAGGGAT30750.074.889834