FastQCFastQC Report
Mon 27 Feb 2023
SRR3126948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621371
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT957383.6522109995113246No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT68940.2629921518167402No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT68880.2627632639561512No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT51920.198064295362999No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35880.13687494063221117No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC35170.13416643428190822No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG33420.1274905383480629No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC30170.11509244589949306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT288100.088.886691
ATAGGGC15750.074.6012953
AGGGATG34500.071.522675
GAATAGG17050.070.602151
GTACGGG6000.068.930282
GTAGGGA17750.068.578553
AGTAGGG37850.067.79622
AATAGGG37350.067.5713042
ATAGGGA19700.067.516073
AGTAAGG16750.066.532821
TAGGGCA12650.066.508064
AGATAGG15600.066.313271
ATGTAGG7950.066.2450261
GTAAGGG37950.066.131462
ACGGGAT6550.066.017394
GGATAGG17100.065.7211
TAGAGGG45000.065.692652
TAGCGGG14700.065.22162
AGGGAAT26250.065.174185
GTAGGGC11400.065.138713