FastQCFastQC Report
Mon 27 Feb 2023
SRR3126947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066117
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT723983.5040609994496923No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT67460.32650619495410954No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT65270.3159066016106542No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT46580.2254470584192473No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC32010.1549283027050259No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG31530.1526051041639946No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC29710.1437963096959175No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27320.13222871696036573No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC21380.10347913501510321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT221650.089.388661
ATAGGGC10950.074.8836063
GAATAGG11350.072.284881
ACGGGAT5500.071.115954
AGGGATG29350.070.450795
CGTTTCT10650.069.50951
AATAGGG25400.068.835562
TAGGGCA9750.068.150034
AGTAGGG29500.067.895292
ATAGGGA15000.067.860043
TAGAGGG33150.067.3856662
GGTAAGG13200.067.1549451
AGGGACT22550.066.2707445
GTAGGGC10300.065.883533
TAGGGAT11200.065.6384354
GATAGGG27700.065.501872
AGAGGGC27400.065.355883
GTACGGG4000.064.800062
ATAGGGT6550.064.7519453
ATAAGGG26650.064.545862