FastQCFastQC Report
Mon 27 Feb 2023
SRR3126946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127801
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT746093.5063899302613355No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT65030.30562068539304194No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT62010.2914276288055133No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT45930.215856652008341No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC33310.1565465943478737No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29630.13925174393658052No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC29620.13920474706046287No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG29470.13849979391869824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT228300.089.18251
ATAGGGC13300.075.265133
AGGGATG29050.071.505915
GAATAGG11850.070.696951
GATAGGG29200.069.232
AGAGGGC31900.069.0952153
AATAGGG28900.068.484612
TAGGGCA9450.067.635224
AGTAGGG30100.067.4723742
GTAGGGT6850.067.235923
ATAGGGA15800.067.222893
CGTTTCT11500.066.709661
AGGGACT21900.066.524775
TAGCGGG12750.066.369882
ATAGCGG6200.066.042971
TAGGGCG3850.065.917224
ATAGGGT8150.065.737483
TGTAGGG13350.064.795562
AGATAGG12100.064.568661
GTAGGGC8700.064.282613