FastQCFastQC Report
Mon 27 Feb 2023
SRR3126945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946999
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278670.9456060215833124No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36390.12348154851766152No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG30380.1030879209663797TruSeq Adapter, Index 22 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94650.082.3165361
ATAGGGC13600.076.725033
TAGGGCA14550.072.6883244
ACGGGTA2300.071.5295264
AGTAGGG43950.070.6876452
GAATAGG19900.070.412271
GTAGGGC14300.068.696293
AGGGCAT27250.068.1323855
ATAGGGA27100.067.9891743
AATAGGG47350.066.703742
CGTAGGG9050.066.475612
TAGGGTC7500.066.43394
GTAGGGA21800.066.407523
AGGGATG38350.066.183765
TGTAGGG23100.066.1262
ACGGGAT7350.065.871124
AGGGAAT35550.065.8434755
AAGAGGG106900.065.686152
TAGAGGG52700.065.194012
TAAGGGA25600.065.179563
Produced by FastQC (version 0.11.5)