FastQCFastQC Report
Mon 27 Feb 2023
SRR3126943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353748
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213910.9088058704670169No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG28630.12163579108723618TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28470.12095602417930892No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC23950.1017526090303635Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72800.083.0100251
AGTAGGG32750.071.220842
CGTAGGG5650.070.746872
ATAGGGC11650.069.385963
GTAGGGC10600.069.165233
AGGGCAT22800.068.640195
TAGGGCA10500.068.481184
TAGACGG2750.068.4581
AGGGATG32300.067.221565
TAAGGGA19800.066.697613
AAGAGGG85800.066.647372
AGGGAAT26550.066.556795
ATAAGGG31750.066.35462
TAAACGG3500.065.890821
TAGGGTC6350.065.869664
TATAGGG15850.065.8658752
GTAGGGT7450.065.606393
ATAGAGG16250.065.4563751
ACGGGAT6350.065.129554
CGAAGGG26250.064.671752
Produced by FastQC (version 0.11.5)