FastQCFastQC Report
Mon 27 Feb 2023
SRR3126942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573472
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79101.3793175604039953No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG24200.4219909603258747No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG23800.4150159031304057No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC20960.36549299704257576No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8520.14856871826348975No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT8250.14386055465654818No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7820.136362368171419No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT7640.13322359243345797No Hit
GGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT6550.11421656157580493No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT6100.1063696222309023No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG6020.10497461079180849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG550.093.9977346
ACGGGAT1150.089.910874
GTATAGG1050.085.067821
CGTTTTT26450.084.6023251
GTACGGG850.082.939182
GGGCGAT2000.079.898076
AGACGGG3600.078.331442
CGGGTAT1450.077.791235
TTACGGG1150.077.65032
ACGGGTA1100.076.9072344
TAGCGGG2300.075.606872
AGGGCAC3000.075.198195
GTCGATG255.5576686E-575.198187
GTAGAGG2950.074.899141
TAAGCGG1200.074.434351
GGCGATG1400.073.855367
CGAAGGG4950.072.159882
TACGGGG2550.071.8806153
GGTAAGG2800.070.516751
ATAACGG200.002160389770.516751
Produced by FastQC (version 0.11.5)