FastQCFastQC Report
Mon 27 Feb 2023
SRR3126941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471867
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56831.2043647892308638No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG21630.45839187737222564No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG20600.436563692735453No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC19220.40731816380463143No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7950.16847967753625492No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7300.15470460956159257No Hit
GGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7130.15110189947591166No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6830.14474417579529825No Hit
GGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT6040.12800217010301632No Hit
AGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT5810.12312791528121271No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT5430.11507479861910241No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG5100.1080813025704277No Hit
AAAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT5030.10659783371161789No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT4950.10490244073012099No Hit
AGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT4790.10151165476712717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG201.8134533E-594.242141
TACGGGT156.85836E-494.142123
CGCGTTA201.8374987E-593.9924858
CGTTTTT18700.087.186581
ACGGGTA650.086.900414
GTATAGG1250.082.933081
CGAAGGG3250.082.5641562
TTACGGG800.082.3830952
CGAGGGT401.022272E-982.374363
CGGGTAT700.080.564985
GGGTACG353.8618964E-880.564986
AGGGCGA1700.080.170055
ACGGGAT900.078.451774
GGTAAGG1550.076.0017241
CGTAGGG750.075.3216862
GCCGTTG255.5145323E-575.313694
AGTAAGG2450.075.009051
GTACGGG950.074.3306052
TAGCGGG1850.073.79492
TAAGCGG1150.073.7547151
Produced by FastQC (version 0.11.5)