FastQCFastQC Report
Mon 27 Feb 2023
SRR3126940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484005
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58461.2078387619962603No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG20710.4278881416514292No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG20560.4247890001136352No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC18590.3840869412506069No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7870.16260162601626016No Hit
GGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7030.1452464334046136No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7030.1452464334046136No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7000.14462660509705477No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT6080.12561853699858472No Hit
AGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT5810.12004008223055547No Hit
GGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT5770.11921364448714374No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT5110.10557742172084998No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG5060.10454437454158533No Hit
AAAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT5030.10392454623402651No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT4930.10185845187549716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG156.8978796E-494.0065194
TACGGGT201.8371273E-593.99683
CCGTAAG156.900713E-493.9967968
AGGGTAC1200.090.080265
ATAGCGG750.087.857411
CGTTTTT21250.087.266781
GGGTACG650.086.766286
CGTAGGG1200.086.172632
TAGCGGG1700.085.7118152
GACCGAT550.085.451648
ACGGGAT700.080.568684
AGGGCGA1700.080.1737445
ATACCGG301.442686E-678.4441151
ATAGGGC2000.077.5473563
TACGAGG850.077.521251
AGTGCGG1100.077.017861
GCGTAGG551.8189894E-1277.017861
TATGGGT551.8189894E-1276.906473
ACGGGTA551.8189894E-1276.906474
TAGGGAC2100.076.092654
Produced by FastQC (version 0.11.5)