FastQCFastQC Report
Mon 27 Feb 2023
SRR3126939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021810
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT979833.2425268299462906No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT57040.18876104056840107No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT47560.15738911447112822No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT44440.1470641767682283No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39250.1298890400124429No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG36720.12151657450336056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT316200.089.916271
AGGGATG54800.074.101685
ACGGGTA3000.073.632834
ATAGGGC18550.073.220793
TAGGGCA13550.069.719084
AGGGCAT22050.067.7818455
AGTAGGG44350.067.3964162
AGGGACT35550.067.1612855
AGATAGG16900.066.7705151
GAGGGAT36050.066.62094
GGTAAGG16300.066.343811
GTTTTTT433700.066.2102362
GAATAGG16350.066.140931
ATAGGGA26550.065.850483
GTAGGGA22550.065.6514743
ACGGGAT7950.065.622194
TAGAGGG54100.065.587782
GTACGGG6600.065.511452
AGTAAGG19250.065.458231
TAGGGAT21200.065.1787954
Produced by FastQC (version 0.11.5)