FastQCFastQC Report
Mon 27 Feb 2023
SRR3126938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367851
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT733743.0987591702349513No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT54160.22873060847156346No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT44730.18890546744706485No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT42330.1787696945458139No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG32550.13746641997321624No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29090.12285401404057941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT237100.090.122611
AGGGATG42400.074.259395
ATAGCGG5700.071.1375661
AGGGAAT25350.070.259285
AGTAGGG34800.070.009472
GAATAGG13800.069.698991
ATAGGGA20900.069.670423
ACGGGAT6050.069.321974
ATAGGGC13700.069.13723
AGTAAGG13850.069.106941
TAGGGTA7600.068.8249054
TAGAGGG40950.068.5863652
TAGGGAT17250.067.748374
GTAGGGC11700.067.6643143
TAGGGCA11300.067.557394
TATAGGG16250.066.989092
AGGGCAT17800.066.7948465
GAGGGAT29550.066.658434
AGAGGGC34200.065.862473
ACGGGTA2650.065.794884
Produced by FastQC (version 0.11.5)