FastQCFastQC Report
Mon 27 Feb 2023
SRR3126937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525721
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT775763.0714397987742905No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT54250.21479015298997792No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT44560.1764248703637496No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT44260.17523709071587876No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33930.13433787817419263No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG32700.12946798161792217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT249600.089.766081
ACGGGAT6550.078.2103044
AGGGATG41700.073.370875
AGTAGGG36850.071.719222
ATAGGGC13850.070.242393
TAGGGCA12000.069.3219454
ATAGGGA21150.069.108073
TAGGGCG5050.067.937614
AGGGAAT27050.067.239195
GAGGGAT29600.067.162594
GTACGGG5750.067.0629652
ATAAGGG32900.066.893932
AGGGACT30150.066.560915
TATAGGG17650.066.0762
AGAGGGC36200.065.823073
GTAGGGC13500.065.7971043
GTTTTTT344100.065.543742
GTAGGGA18300.064.718473
AGTAAGG15500.064.6841351
AAGAGGG78750.064.612042
Produced by FastQC (version 0.11.5)