FastQCFastQC Report
Mon 27 Feb 2023
SRR3126936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955069
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3032573.812122811254057No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT433170.5445207326297232No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT141880.17835168997277082No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT126510.15903067591242764No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT111730.1404513273234965No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT110860.13935768501819407No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT109680.13787435407537005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT917250.090.626721
ATAGGGC41000.071.9068763
AGGGACT184850.071.363575
GGTAAGG47650.069.425591
GTTTTTT1212700.068.971912
AGTAGGG112100.068.3120962
ATAGAGG52350.066.703231
GGATAGG43900.066.6610951
GTAGGGA50000.065.6512153
TAGGGCA28850.065.297134
AAGGGGC256300.065.19514
TAGGGAC36000.065.148854
AGATAGG40900.064.407121
TAGAGGG146850.064.398012
ATAGGGA50900.064.305333
CAGGGAC169950.064.207364
TATAGGG54700.064.057252
AGGGGCT313000.063.6011855
TAGTAGG25350.063.5760421
GTAGGGT20050.063.4204523
Produced by FastQC (version 0.11.5)