FastQCFastQC Report
Mon 27 Feb 2023
SRR3126933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9459152
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3499593.6996868218208143No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT526300.5563923700560051No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT152000.16069093720029026No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT143850.15207494287014311No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT136750.1445689846193401No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT124280.13138598470560572No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT121490.12843646026620567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1058850.090.329381
ATAGGGC47700.071.363193
AGGGACT230200.071.179315
GGTAAGG53150.070.9969941
AGTAGGG133250.069.8329242
GTTTTTT1409400.068.531472
TAGGGCA34800.067.283524
AAGGGGC312850.066.141484
ATAGGGA58400.065.775363
AATAGGG119300.065.701722
TAGGGAC41200.065.504834
AGATAGG44300.065.319111
GTAGGGA61200.065.2244343
AGGGGCT367650.063.9939735
GATAGGG127500.063.910172
CAGGGAC210700.063.9097484
TATAGGG67400.063.9024052
ATAAGGG119950.063.620912
TAGTAGG34400.063.6009141
GGATAGG54450.063.5123631
Produced by FastQC (version 0.11.5)