FastQCFastQC Report
Mon 27 Feb 2023
SRR3126931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7285538
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2098252.8800206655980656No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT354610.4867313848338997No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT237040.3253568919687194RNA PCR Primer, Index 16 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC212410.2915501916262052No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT199580.2739399616061298No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT180720.2480530607348421No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG179230.2460079132110765RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC117290.16099016984058007RNA PCR Primer, Index 16 (96% over 25bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT100170.13749156205073668No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT97050.13320910549090542RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC91940.126195210291951No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC91080.12501478957353596RNA PCR Primer, Index 16 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT90770.12458928908201426No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC73500.1008847939575636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT681750.090.664761
ATAGGGC35100.073.912533
AGGGACT160050.071.765665
GGTAAGG40550.071.216351
AGTAGGG101100.070.242412
GTTTTTT906300.068.581952
TAGTAGG24850.067.568461
TAGGGCA24450.067.468254
AGATAGG36800.067.098631
ATAGGGA38950.066.727333
GGATAGG39050.066.4844741
AGTAAGG41950.066.373071
AAGGGGC241800.066.18084
AATAGGG81350.065.861762
ATAGAGG44350.065.750681
TAGGGAC28450.065.2508244
GTAAGGG105350.065.178112
GATAGGG95550.064.87842
TATAGGG46800.064.573042
ATAGCGG14350.064.567881
Produced by FastQC (version 0.11.5)