FastQCFastQC Report
Mon 27 Feb 2023
SRR3126930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10655760
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3238083.0388071803419No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT593970.557416833712471No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT245400.2302979796842271RNA PCR Primer, Index 16 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT191670.17987454672402534No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG183620.17231994714595675RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT175380.16458704024865425No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT170950.16042966433177924No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT153860.1443913901964759No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC149220.14003693776886866No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC120650.11322514771353709RNA PCR Primer, Index 16 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT106620.10005855987747472RNA PCR Primer, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1053200.090.382061
ATAGGGC54300.071.922233
AGGGACT250500.071.554175
AGTAGGG157250.069.399842
GGTAAGG58850.069.245271
GTTTTTT1408900.068.22422
ATAGAGG70300.067.672861
GGATAGG58850.067.006391
TAGGGAC45600.066.062534
TATAGGG77650.065.941742
AAGGGGC352450.065.510554
GTATAGG32900.065.507091
AGATAGG51900.065.461891
GATAGGG140600.064.575972
AGTAAGG63000.064.310411
ATAGGGA62550.064.164173
CAGGGAC232300.064.111374
TAGTAGG36600.064.0275651
GTAGGGC46700.064.003283
ATAGCGG24500.063.7662241
Produced by FastQC (version 0.11.5)