FastQCFastQC Report
Mon 27 Feb 2023
SRR3126929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642300
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3933373.1112772201260843No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT708280.5602461577402845No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT240300.190076172848295RNA PCR Primer, Index 16 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT207600.16421062623098645No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT207320.16398914754435506No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG204310.16160825166306764RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT196990.1558181659982756No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT186120.1472200469851214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1247800.090.395191
ATAGGGC61100.073.2573
AGGGACT312500.069.871335
AGTAGGG183200.069.17082
GTTTTTT1670700.068.075522
GGTAAGG71500.067.39041
ATAGAGG81850.066.974791
TATAGGG85950.066.469122
TAGTAGG43750.066.468291
AAGGGGC413350.065.780264
AGATAGG66900.065.693871
ATAGGGA79900.065.3175053
AATAGGG159150.065.057922
TAGGGAC58600.064.7494664
TAGGGCA44900.064.60994
ATAGCGG26400.064.34391
AGTAAGG73450.064.321
AGGGGCT507400.063.974545
GGATAGG72600.063.9712221
TAGAGGG235300.063.746782
Produced by FastQC (version 0.11.5)