FastQCFastQC Report
Mon 27 Feb 2023
SRR3126928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323043
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77730.587509249510409No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44630.33732841638555966No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38730.2927342497560548No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG30180.2281104998099079No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG29090.21987191648344004No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT28450.21503458315413784No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG25770.1947782498376848No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC24610.1860105831783245Illumina Single End Adapter 1 (95% over 21bp)
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA13340.1008281665826432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT700.093.984738
AGGGCGC3300.086.864685
CGTTTTT33600.084.66731
AGGGATG26550.081.064055
CGCTACG7350.080.8271563
AGTAAGG8000.080.791981
TAGGGCG2700.080.3282244
AGTACGG1600.079.612531
TAACGGG3850.079.605522
CTACGAC7550.079.046765
ATACCGG301.4239322E-678.629661
ACGGGTC1500.078.5819554
GGATGGC18250.078.535197
TCCGCTA7650.078.3213041
GGTAAGG7150.077.8598561
AGGGCGA5500.077.751015
TAAACGG1400.077.506381
AAGGGCA16050.076.9662554
CGAAGGG11250.076.69892
GCTACGA7750.076.6554264
Produced by FastQC (version 0.11.5)