FastQCFastQC Report
Mon 27 Feb 2023
SRR3126926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374599
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76730.5581991548080567No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44530.3239490207689661No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41220.29986927096556887No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG31360.22813926097720133No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG30820.22421084258027252No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27390.1992581109108911No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG27290.19853062602257096No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC27240.1981668835784109Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT1050.093.989278
CGTTTTT32800.083.3830641
AGGGATG25950.082.036885
CGCTACG7600.081.622263
AGTAAGG8900.080.9239351
CGAAGGG11150.080.080542
TCCGCTA7800.079.662681
AAGGGAC12700.079.187814
GGTAAGG7850.079.1552661
GCTACGA7900.078.5226754
AAGGGTA7800.078.3243944
TAGGGCG2700.078.324394
AGGGTAC3800.077.912165
AGGGCGT1750.077.876825
CTAGCGG850.077.532661
TAGCGGG5250.076.981692
AGGGCGC3000.076.7579045
CGTAGGG4900.076.725932
ACGGGTC1350.076.583854
AGGGCAT12550.076.389695
Produced by FastQC (version 0.11.5)