FastQCFastQC Report
Mon 27 Feb 2023
SRR3126925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463798
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157840.6406369353331726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49660.20155873168173688No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38260.15528870467465272No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT34020.1380795016474565No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG32430.1316260505122579TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC30320.12306203674164846Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG29630.1202614824754302TruSeq Adapter, Index 23 (95% over 23bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29200.11851620952691738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59050.082.797081
CGTAGGG6050.077.15532
ATAGCGG4550.074.678631
TAGGGCA12550.074.013074
ACGGGTA3600.073.345154
AGTAAGG15950.072.490421
ATAGGGC17500.071.9381263
AGTACGG3500.071.4632951
AGGGCAT23350.071.04235
GTAGGGT9150.070.596863
GGTAAGG15850.069.970311
GAATAGG15300.069.709531
TAGAGGG42550.069.479062
TAAGGGA19050.069.302513
AGTAGGG39700.069.2411042
CGAAGGG20900.068.808082
AGAGGGT22500.068.734893
AGGGTAA10350.068.559215
ATTGCGG3650.068.526441
AGAAGGG79750.068.523362
Produced by FastQC (version 0.11.5)