FastQCFastQC Report
Mon 27 Feb 2023
SRR3126924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464426
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151350.6141389516260581No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47860.1942034372304139No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37180.15086677384510633No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG33400.13552851657951992TruSeq Adapter, Index 23 (95% over 23bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT32430.13159250876268958No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC31610.12826516194846183Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG29370.11917582430959583TruSeq Adapter, Index 23 (95% over 23bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27870.11308921428356948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54750.083.7237851
CGTAGGG7200.080.9860842
ACGGGTA2900.076.1683044
ATAGCGG4550.075.50671
ATAGGGC14300.074.604373
ACTAGGG11200.074.3149952
GCGTAGG4000.074.1232761
TAGGGTC7600.072.969744
AGTAGGG40850.071.946422
TGTAGGG17200.071.356452
TAGGGCA12450.070.9680564
GGTAAGG14150.070.843041
AAGGGTA17000.070.772654
AGGGCAT22350.070.443635
AGGGTAC9500.069.754135
TAGGGCG5150.069.3554844
GTAGGGT9200.068.963673
TATAGGG19900.068.52772
AGGGATG27800.068.298475
AGAAGGG76050.067.954732
Produced by FastQC (version 0.11.5)