FastQCFastQC Report
Mon 27 Feb 2023
SRR3126923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595175
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159910.6161819530474824No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49500.19073858217653916No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37870.14592464862677854No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG35240.13579045729093414TruSeq Adapter, Index 23 (95% over 23bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT34230.13189861955359464No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC33660.12970223588004662Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG31760.12238095696821988TruSeq Adapter, Index 23 (95% over 23bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29010.11178436906952324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63150.081.289261
ACGGGTA3300.075.477294
CGTAGGG6400.074.164442
ATAGGGC17200.074.044913
AGTAGGG43650.073.103782
AGGGCAT24250.072.479335
GAATAGG16050.072.4108661
ATAGCGG5050.070.811531
TAGGGCA13100.070.313564
AGTAAGG18250.069.86961
TAGAGGG45600.069.565392
GGTAAGG14750.069.5418551
AAGGGTA19000.069.503564
TAGGGCG4400.069.424864
AGGGTAC9950.068.957915
GTAGGGC10850.068.8686753
CGAGGGA12450.068.699943
AGAGGGT25300.068.1710363
GTAGGGT9900.067.882093
ACGGGAT7200.067.882084
Produced by FastQC (version 0.11.5)