FastQCFastQC Report
Mon 27 Feb 2023
SRR3126922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989172
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207911.0452087602278737No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC58420.29369003786500114No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG51800.26040985897649876No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG49690.24980243035795802No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT42890.21561735234559906No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40750.2048591072064155No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29760.1496099884776178No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT22070.1109506870195237No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT21860.1098949713750244No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20470.10290713925190985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73300.085.094611
AGGGCAT18250.078.281175
TAGGGCA10300.078.2204744
AGGGTAC8300.075.3043755
AGAGGGT18000.074.337413
AGAGGGC24300.073.872073
ACGGGAT4350.073.6514364
GAGGGTA9600.073.6175844
CGTAGGG5250.072.6921162
TAAGGGA14650.072.682923
AAGGGTC11500.072.5165254
GGTAAGG11400.072.3734741
TAGGGTA7250.072.1350864
GTAGGGC8300.071.524373
AGGGAAT26900.071.452545
TAGAGGG33000.071.243982
TAGCGGG8550.071.0861742
TGTAGGG12450.070.767492
ATAGCGG3200.070.719221
AGTAAGG10300.070.490361