FastQCFastQC Report
Mon 27 Feb 2023
SRR3126921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992916
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198100.9940208217506408No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC61810.31014854615046494No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG53920.27055831756080034No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG50320.2524943349343374No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT43770.21962792210007848No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39120.1962952778742305No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29420.14762288024181652No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT22500.11289989141539333No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT22320.11199669228407017No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20370.10221203502806943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70450.085.817561
CGTAGGG4800.077.394642
AGGGCAT17700.076.2068255
GGGTACG1550.075.8042456
GCATAGG5150.073.0864261
AGGGAAT25300.072.8200155
AAGGGTA13800.071.860234
GTACGGG4450.071.8577962
AGGGTAT10450.071.509885
AAGAGGG60100.071.28022
GGTAAGG11400.070.986791
AAGGGTC12050.070.595454
CGAGGGA9250.070.1168753
AAGGGCA28650.069.882784
ACGGGTA2300.069.4762344
TAGGGCA8600.069.404954
AGTAAGG11400.068.9232251
AGAGGGC24350.068.9055943
AGGGTAA8200.068.778495
ATGAGGG28450.068.760032