FastQCFastQC Report
Mon 27 Feb 2023
SRR3126919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215423
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654592.95469533357738No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT76210.3439975119875527TruSeq Adapter, Index 27 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG59770.26979046439438426TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC55460.25033594035992224TruSeq Adapter, Index 27 (100% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT39720.17928856024334855No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT33720.15220569615825058No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG25290.11415427211868795TruSeq Adapter, Index 27 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25010.11289040512805004No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22750.1026891929893298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT216800.090.161641
AGGGATG39950.075.517235
ACGGGAT6950.074.63424
TAGGGCG3850.073.48874
GTAGGGC10500.072.7538153
AGGGAAT27050.072.095575
AGTAGGG35250.071.570582
AGTACGG3100.071.532321
ACGGGTA1850.071.370114
AGGGCAT17900.070.8824165
TAGAGGG38850.070.7648242
AGTAAGG12250.070.4824751
ATAGGGC12750.069.900723
CGTAGGG5600.069.8923952
GAGGGAT27200.069.519324
GAATAGG13250.069.4360351
TAGGGTA6800.069.3459554
AAGGGAT39600.069.1848454
AGAGGGC30550.069.150743
AAGGGTA13450.068.716944
Produced by FastQC (version 0.11.5)