FastQCFastQC Report
Mon 27 Feb 2023
SRR3126918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215843
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT626542.827546897501312No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT75270.33969013147592136TruSeq Adapter, Index 27 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG60100.2712286023874435TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC55290.24952128828621883TruSeq Adapter, Index 27 (100% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT40300.18187209111836894No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT31910.14400839770687723No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25180.11363620978562108No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG24450.1103417525519633TruSeq Adapter, Index 27 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22240.10036812174869789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT218600.089.93861
AGGGATG38550.077.904235
GAGGGAT28800.074.0884
AGTAAGG12000.073.740891
ATAGCGG3250.072.413321
AGGGAAT28250.071.537625
ATAGGGA21000.071.393043
AGTAGGG30200.071.162082
AGAGGGC27500.070.9250953
AGGGTAC9450.070.622175
AGGGTAG8150.070.353675
AAGGGAT39100.070.1973344
AAGGGTA14150.070.082664
TGTAGGG15300.069.770812
AGGGCAT18000.068.931215
TAGGGCG3350.068.7441564
ACGGGAT7250.068.7151264
TAGAGGG38850.068.1485752
GTAGGGT6150.068.014173
GAATAGG13150.068.007941
Produced by FastQC (version 0.11.5)