FastQCFastQC Report
Mon 27 Feb 2023
SRR3126917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314210
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT653372.8232960707973778No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT79810.3448693074526512TruSeq Adapter, Index 27 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG65850.28454634626935327TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC58580.2531317382605727TruSeq Adapter, Index 27 (100% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT42040.18166026419382855No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT34480.14899252876791647No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG26580.11485560947364327TruSeq Adapter, Index 27 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24550.10608371755372244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT229400.089.660861
AGGGATG37500.078.076145
ACGGGAT6650.075.617734
TAGGGCA12000.074.802084
GAGGGAT27700.073.972154
GAATAGG15250.071.5869141
GGTAAGG13250.071.383141
AGGGAAT28800.070.8204655
TAGGGCG4250.070.770554
AGTAGGG33250.070.670782
GTAGGGC10900.070.278533
AAGGGAT38550.070.21984
ATAGGGC14600.070.172213
AAGGGTA13350.070.054074
AGGGCAT18800.069.994145
TAGCGGG10750.069.947632
TAGAGGG42600.069.721862
AGTAAGG11950.069.698081
AGGGTAC9100.069.2035
CGTAGGG6400.069.0254752
Produced by FastQC (version 0.11.5)