FastQCFastQC Report
Mon 27 Feb 2023
SRR3126914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470361
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76890.5229328035768086No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62810.4271740069275504No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49680.33787620863175777No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG35810.24354563267116033TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG32010.21770163925729805TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC30250.20573178967614075TruSeq Adapter, Index 20 (95% over 23bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23660.1609128642557848No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT19930.13554494440480944No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA17670.12017456937445975No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT16360.11126519269757562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT6150.086.347514
CGTTTTT37800.083.919881
AGGGATG34200.083.136275
TCCGCTA11850.082.214571
GCTACGA11950.081.797854
CGCTACG11850.081.694983
GCGAAGG5100.081.209961
GGATGGC22500.080.624317
TAGGGCG2450.080.56194
AGGGCAT13900.080.129945
TAGGGCA8000.079.3031164
AGGGCAA12150.079.2937245
AAGGGCA19550.078.8448944
AACGGGA7200.078.3240663
GAACGGG8400.078.3240662
CTACGAC12500.078.201415
CGAAGGG11800.078.058562
AGCGGGA9600.077.834543
AATGCGG3100.077.429291
GCACCGA850.077.4052358