Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126914.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1470361 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7689 | 0.5229328035768086 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 6281 | 0.4271740069275504 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 4968 | 0.33787620863175777 | No Hit |
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG | 3581 | 0.24354563267116033 | TruSeq Adapter, Index 23 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG | 3201 | 0.21770163925729805 | TruSeq Adapter, Index 23 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC | 3025 | 0.20573178967614075 | TruSeq Adapter, Index 20 (95% over 23bp) |
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG | 2366 | 0.1609128642557848 | No Hit |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 1993 | 0.13554494440480944 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA | 1767 | 0.12017456937445975 | No Hit |
AAAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT | 1636 | 0.11126519269757562 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGGGAT | 615 | 0.0 | 86.34751 | 4 |
CGTTTTT | 3780 | 0.0 | 83.91988 | 1 |
AGGGATG | 3420 | 0.0 | 83.13627 | 5 |
TCCGCTA | 1185 | 0.0 | 82.21457 | 1 |
GCTACGA | 1195 | 0.0 | 81.79785 | 4 |
CGCTACG | 1185 | 0.0 | 81.69498 | 3 |
GCGAAGG | 510 | 0.0 | 81.20996 | 1 |
GGATGGC | 2250 | 0.0 | 80.62431 | 7 |
TAGGGCG | 245 | 0.0 | 80.5619 | 4 |
AGGGCAT | 1390 | 0.0 | 80.12994 | 5 |
TAGGGCA | 800 | 0.0 | 79.303116 | 4 |
AGGGCAA | 1215 | 0.0 | 79.293724 | 5 |
AAGGGCA | 1955 | 0.0 | 78.844894 | 4 |
AACGGGA | 720 | 0.0 | 78.324066 | 3 |
GAACGGG | 840 | 0.0 | 78.324066 | 2 |
CTACGAC | 1250 | 0.0 | 78.20141 | 5 |
CGAAGGG | 1180 | 0.0 | 78.05856 | 2 |
AGCGGGA | 960 | 0.0 | 77.83454 | 3 |
AATGCGG | 310 | 0.0 | 77.42929 | 1 |
GCACCGA | 85 | 0.0 | 77.405235 | 8 |