FastQCFastQC Report
Mon 27 Feb 2023
SRR3126913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130494
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56380.49872002858927156No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44520.39381013963806977No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG27520.24343340168103503No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG26350.23308394383340383No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC25380.22450362407938476No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25270.22353059812789808No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22330.19752426815179913No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18860.16682972222762793No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA17510.15488804009574575No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT11970.10588291490268856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGC2650.090.439725
ACGGGTA1200.090.350014
CGTAGGG2350.090.2744452
CGTTTTT29550.086.374921
ACGACGG550.085.779841
CGCTACG3650.085.237893
GACCGAT950.084.093088
AGTAAGG7100.083.725951
CGAAGGG9650.083.050412
GTTACGG800.082.563091
AGGGATG18850.082.518555
ACGGGAT3950.082.3443154
GCTACGA3800.081.873244
GGGCGAT3750.081.4548656
CGAGGGA6050.081.032563
TAGGGCG2000.080.136534
CTACGAC3900.079.526935
AGGGCAT9900.079.271345
GTAGGGT2800.079.126413
AACGAGG3700.079.056551